Effects of Incubation Time and Injection Sites on Gene Transfection Efficiency in Chicken Embryos by In Ovo Lipofection
نویسندگان
چکیده
منابع مشابه
Gene transfer into older chicken embryos by ex ovo electroporation.
The chicken embryo provides an excellent model system for studying gene function and regulation during embryonic development. In ovo electroporation is a powerful method to over-express exogenous genes or down-regulate endogenous genes in vivo in chicken embryos(1). Different structures such as DNA plasmids encoding genes(2-4), small interfering RNA (siRNA) plasmids(5), small synthetic RNA olig...
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the effects of time planning and task complexity on accuracy of narrative task performance
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Effects of In-ovo Injection of Different Nutrients on the Hatchability and growth Performance in Broilers
The aim of this study was to investigate the effects of in ovo injections of albumin, ami-no acid and dextrose into the amnion on the hatchability, growth performance and slaughter yield of Ross 308 broiler chicks. Fertile eggs (n=360) were assigned to 6 treatment groups (4 replicates of 15 eggs each) including: non-injected eggs (control), and eggs injected with 0.7 ml distilled water (sham), ...
متن کاملEx ovo electroporation for gene transfer into older chicken embryos.
In ovo electroporation is an excellent method to ectopically induce or inhibit gene expression in chicken embryos and to study the in vivo function of genes during embryonic development. However, the application of electroporation in ovo to date is limited to an early stage of incubation (< stage 20). In older embryos (> stage 22), the vitelline and allantoic vessels have developed extensively ...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 1996
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.67.882